This special issue launched by CNHUPO (HUPO in China) reviews the major advances in proteomics from 2001 (HUPO initiation) to 2021 (the 20th anniversary of HUPO). Jiaqi Zhang wrote the draft. I reviewed the scientific content. Permission to reproduce and modify the issue was granted. The original version (Chinese) could be referenced here. You could use the Language Switch icon at the top right of this page for assisted reading. The Original Title: John Fenn 躬身入局|生物大分子分析领域的变革 1迷茫 | 对科研失去兴趣 拥有坚固家…read more
分类： 科普
Overview of Proteomics Standards Initiative, Part One
This special issue launched by CNHUPO (HUPO in China) reviews the major advances in proteomics from 2001 (HUPO initiation) to 2021 (the 20th anniversary of HUPO). Jiaqi Zhang wrote the draft. Shanjun Chen, Junhong Xiao reviewed the language. I reviewed the scientific content. Permission to reproduce and modify the issue was granted. The original version (Chinese) could be referenced here. You could use the Language Switch icon at the top right of this page for assisted reading. The original…read more
A review for Human Plasma Proteome Project (HPPP)
This special issue launched by CNHUPO (HUPO in China) reviews the major advances in proteomics from 2001 (HUPO initiation) to 2021 (the 20th anniversary of HUPO). Jiaqi Zhang wrote the draft and I reviewed the scientific content. Permission to reproduce and modify the issue was granted. The original version (Chinese) could be referenced here. You could use the Language Switch icon at the top right of this page for assisted reading. The original title: 等待蝶变 ｜人类血浆蛋白质组项目(HPPP)研究进程 Protein…read more
Proteomics: Boom of the post-genomic era
This special issue launched by CNHUPO (HUPO in China) reviews the major advances in proteomics from 2001 (HUPO initiation) to 2021 (the 20th anniversary of HUPO). Jiaqi Zhang drafted the content and I helped with scientific revision. Permission to reproduce the issue was granted. The formatted version (Chinese) could be referenced here.You could use the Language Switch icon at the top right of this page for assisted reading. The original title: 后基因组时代的高歌猛进 | HUPO的启航 拿起笔，画一条竖线，将世界分为宏观和微观；再画一条横线，将…read more
五千万个西湖 Fifty Million Westlakes
如果一个蛋白是一滴水，那么我们每个人的体内，都蕴藏着五千万个西湖。If we see a protein as a tiny drop of water, then each one of us amounts to fifty million westlakes. 一个人~5E13细胞 (Google)A man ~ 5E13 cells 一个人~2E4种蛋白 (Swissprot)A man ~ 2E4 types of proteins 每个细胞每种蛋白拷贝数中位值 1.8E4 (PMID22068331)Median value of copy number per protein per cell = 1.8E4 一个拷贝~一滴水(0.04ml)~2.8E-15西湖One copy ~ One waterdrop (0.04ml) ~ 2.8E-15 Westlakes Thus一个人~1.8E22蛋白拷贝~5E7西湖A man ~ 1.8E22 protein copies ~ 5E7 Westlakes
蛋白质组：蛋白质的4+级结构（下）
原标题：蛋白质是什么？（下） 蛋白质组：蛋白质的4+级结构（上）蛋白质组：蛋白质的4+级结构（中） 我们前面讲了蛋白质的4级与4+级结构，大家应该对这张图有很直接的理解了。每个点是一个蛋白，每条线是蛋白之间的关系，每一簇是一个蛋白的4+结构和或蛋白质网络。人的身体里面有不同的网络、它们相互联系以组成这个有活力的生命体。 我们上面从蛋白质的简单讲到复杂，而蛋白质的酶解可以被认为是的对这个过程的反向：把一个线团给拆成线、然后再用剪刀这条线剪断，就是蛋白质酶解的过程。 我们在认识一个问题之前，要了解我们做它的目的；蛋白质为什么要酶解呢？在自然界里蛋白质酶解是无时无刻不在发生的，因为蛋白质是我们人体的营养来源之一，我们喝蛋白粉、我们吃肉，都是补给自己、供应能量。猪、牛的蛋白质不能被我们直接的利用，而要首先通过酶解转变成小分子多肽或短肽、乃至氨基酸，才能被我们吸收利用；再通过前述的过程搭建我们人体的蛋白质。蛋白质的酶解，英文的意思叫蛋白质的消化，是很好理解的。这个过程也是通过若干种蛋白酶的协同的相互作用去进行的。 我们现在看如何对蛋白质进行酶解。我们是用一个类似于小剪刀的东西把这个线段给剪开；这…read more
