Is MS1 utilized in DIA-MS? During a DIA-MS experiment, MS1 scans are usually acquired. For the TOF-based DIA-MS experiments, MS1 scans are usually of moderate resolution. For example, the original SWATH-MS paper (PMID: 22261725) by Gilet et al used 250-ms to finish a DDA-MS1 scan while 100-ms to finish a SWATH-MS1 on a TOF-MS system. Considering that each scan, regardless of MS1 or MS2 in SWATH, takes 0.1 s, SWATH-MS1 takes around 3.3% of the cycle time. The authors did, however, additionally de…read more
分类： review
How did John Fenn start his career?
This special issue launched by CNHUPO (HUPO in China) reviews the major advances in proteomics from 2001 (HUPO initiation) to 2021 (the 20th anniversary of HUPO). Jiaqi Zhang wrote the draft. I reviewed the scientific content. Permission to reproduce and modify the issue was granted. The original version (Chinese) could be referenced here. You could use the Language Switch icon at the top right of this page for assisted reading. The Original Title: 诺奖得主John Fenn | 科研之路起航 在生命运作的微观场景中，核酸是细胞生命…read more
Overview of Proteomics Standards Initiative, Part One
This special issue launched by CNHUPO (HUPO in China) reviews the major advances in proteomics from 2001 (HUPO initiation) to 2021 (the 20th anniversary of HUPO). Jiaqi Zhang wrote the draft. Shanjun Chen, Junhong Xiao reviewed the language. I reviewed the scientific content. Permission to reproduce and modify the issue was granted. The original version (Chinese) could be referenced here. You could use the Language Switch icon at the top right of this page for assisted reading. The original…read more
A review for Prof. Nikolai Slavov’s SCP talk
Note: This is a Chinese review for a recent talk of Prof. Nikolai Slavov, focusing on the recent advances of single cell proteomics developed by his lab. Yingqiu and Jiaqi helped with drafting and revising, respectively. The formatted version (Chinese) could be referenced here. You could use the Language Switch icon at the top right of this page for assisted reading. Original title: YIPA研讨会 | Nikolai Slavov 探讨单细胞蛋白组学前沿技术 Talk Theme: Accessible methods for high-throughput, deep and accurate singl…read more
Liquid Chromatography For Trace Proteomics: A Mini-review
Please note that authorship is reserved, and you must have my consent before reproducing this article. Technology has been a main driven power prompting development of system biology study. In the acquisition of proteomic digital data, a separation method in conjugation with tandem mass spectrometry is an optimal combination given that mass spectrometry cannot handle thousands of peptides flowing inside at the same time. The following content presents a mini-review for several separati…read more
