Is MS1 utilized in DIA-MS? During a DIA-MS experiment, MS1 scans are usually acquired. For the TOF-based DIA-MS experiments, MS1 scans are usually of moderate resolution. For example, the original SWATH-MS paper (PMID: 22261725) by Gilet et al used 250-ms to finish a DDA-MS1 scan while 100-ms to finish a SWATH-MS1 on a TOF-MS system. Considering that each scan, regardless of MS1 or MS2 in SWATH, takes 0.1 s, SWATH-MS1 takes around 3.3% of the cycle time. The authors did, however, additionally de…read more
标签： mass spectrometry
How did John Fenn continue his career?
This special issue launched by CNHUPO (HUPO in China) reviews the major advances in proteomics from 2001 (HUPO initiation) to 2021 (the 20th anniversary of HUPO). Jiaqi Zhang wrote the draft. I reviewed the scientific content. Permission to reproduce and modify the issue was granted. The original version (Chinese) could be referenced here. You could use the Language Switch icon at the top right of this page for assisted reading. The Original Title: John Fenn 躬身入局|生物大分子分析领域的变革 1迷茫 | 对科研失去兴趣 拥有坚固家…read more
How did John Fenn start his career?
This special issue launched by CNHUPO (HUPO in China) reviews the major advances in proteomics from 2001 (HUPO initiation) to 2021 (the 20th anniversary of HUPO). Jiaqi Zhang wrote the draft. I reviewed the scientific content. Permission to reproduce and modify the issue was granted. The original version (Chinese) could be referenced here. You could use the Language Switch icon at the top right of this page for assisted reading. The Original Title: 诺奖得主John Fenn | 科研之路起航 在生命运作的微观场景中，核酸是细胞生命…read more
John Yates and his enthusiasm for mass spectrometry
This special issue launched by CNHUPO (HUPO in China) reviews the major advances in proteomics from 2001 (HUPO initiation) to 2021 (the 20th anniversary of HUPO). Jiaqi Zhang drafted the content and I helped with scientific revision. Permission to reproduce and modify the issue was granted. The original version (Chinese) could be referenced here. You could use the Language Switch icon at the top right of this page for assisted reading. The original title: John Yates | 质谱的狂热爱好者 回顾质谱的百年发展史，得益于机械、电…read more
A review for Prof. Nikolai Slavov’s SCP talk
Note: This is a Chinese review for a recent talk of Prof. Nikolai Slavov, focusing on the recent advances of single cell proteomics developed by his lab. Yingqiu and Jiaqi helped with drafting and revising, respectively. The formatted version (Chinese) could be referenced here. You could use the Language Switch icon at the top right of this page for assisted reading. Original title: YIPA研讨会 | Nikolai Slavov 探讨单细胞蛋白组学前沿技术 Talk Theme: Accessible methods for high-throughput, deep and accurate singl…read more
Bibliometric Analysis of Single Cell Proteomics: 2012-2022
Note: The analyses were originated from citexs.com and I made several adjustments on the layout and content. For academic use only. 1.摘要本文基于PubMed文献数据库，使用citexs赛特新思数据分析平台，对2012-01至2022-12关于 single cell 和 proteomics 研究进行文献分析及大数据挖掘，梳理该领域的发展历程，分析研究主题的热点变化等，将分析结果可视化，旨在对single cell 和 proteomics相关领域研究进行系统综述和趋势展望，探讨当前的研究动态及热点，挖掘分析尚需改进的内容，以期为后续研究提供参考。 2.数据来源（1）数据来源于PubMed数据库（2）时间跨度：2012-01至2022-12（3）文献类型：article（4）语种：English（5）共检索到 1443 篇文献（6）检索词：(single cell) AND (proteomics) 3.研究方法本文采用文献计量学方法，以PubMed文…read more
